Coding
Taq Ligase

Part:BBa_K3271026:Design

Designed by: George Liu and Setti Belhouari   Group: iGEM19_uOttawa   (2019-10-16)


Taq Ligase in pSB1K3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 92
    Illegal BamHI site found at 633
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 142
    Illegal SapI.rc site found at 292


Design Notes

Coding sequence was codon-optimized for expression in E. coli and for the removal of illegal restriction sites.


Source

The coding sequence was derived from the genome of T5 phage.

Sequence for original sequence (prior to our adjustments) was obtained from UniProtKB - B7A6G7 (B7A6G7_THEAQ).


References