![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Taq Ligase
Part:BBa_K3271026:Design
Designed by: George Liu and Setti Belhouari Group: iGEM19_uOttawa (2019-10-16)
Taq Ligase in pSB1K3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 92
Illegal BamHI site found at 633 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1026
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 142
Illegal SapI.rc site found at 292
Design Notes
Coding sequence was codon-optimized for expression in E. coli and for the removal of illegal restriction sites.
Source
The coding sequence was derived from the genome of T5 phage.
Sequence for original sequence (prior to our adjustments) was obtained from UniProtKB - B7A6G7 (B7A6G7_THEAQ).